NGS Course: RNA-seq data analysis

Transcriptomics and Single Cell RNA-Seq Analysis

The 2018 edition of the RNA seq data analysis course was organised as a Summerschool together with VIB, ELIXIR Netherlands and ELIXIR Belgium.

The information below concerns the courses as organised before 2018.

Course coordinators

Peter-Bram ‘t Hoen (LUMC), Leon Mei (LUMC), Jan Oosting (LUMC), Szymon Kielbasa (LUMC) and Celia van Gelder (BioSB)

Course description

The BioSB research school and partner LUMC are organizing a 3-day course on RNA-seq data analysis. This is an advanced course for people with experience in NGS. The course will consist of lectures, Galaxy and R practicals and will cover the analysis pipelines for differential transcript expression and variant calling. Examples will be taken from human and mouse studies. The course does not cover prokaryotic RNA profiling nor plant- and metagenomics aspects.

Course topics

  • RNA-seq experimental approaches and study design
  • Quality control and alignment
  • Statistics for differential gene expression
  • eQTL analysis and allele specific expression
  • Single cell RNA sequencing
  • Fusion transcript detection
  • Small RNA profiling
  • Software for RNA-seq data analysis

Target audience

Participants for the RNA-seq course should preferably have participated in the general NGS course or otherwise have demonstrated hands-on experience with NGS data analysis. The course is aimed at PhD students and post-docs, but scientific programmers and data analysts with a background in biology and bioinformatics may also attend.

Information about previously organised courses can be found on the course archive page.