From 26 – 28 September 2016 the 6th edition of the RNA-seq data analysis course is organised in Leiden. The course aims at PhD students, postdocs, and senior researchers who are interested in, planning, or already working with next-generation sequencing. We welcome researchers from both the genomics and bioinformatics fields. Currently available technologies as well as hardware and software solutions will be presented and discussed. The focus of the course will be on the data and ways to analyse these.